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KMID : 0613820020120010067
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Journal of Life Science
2002 Volume.12 No. 1 p.67 ~ p.76
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The increased GUS gene inactivation over generation in Arabidopsis transgenic lines
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Park Soon-Ki
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Abstract
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The effect of transgene inactivation in T2, T3 and F2 generations was analyzed in progeny seedlings which had been generated by Agrobacterium (LBA4404/pBI121)-mediated transformation in Arabidopsis thaliana. In a system which investigated in the expression of β-glucuronidase(GUS)gene in kanamycin-resistant (kanR)seedlings, GUS inactivated seedlings were observed in 5 of 12 tested lines of T2 generation and the frequency of GUS inactivation was approximately 2.3%. Lines with multi-copies of T-DNA exhibited severe GUS gene inactivation with the frequency of 5.8% in T2 generation. In T3 generation lines exhibited GUS gene inactivation with the frequency of 1.3%. In contrast, inactivation increased dramatically up to 12.6% in multi-copy T-DNA line. A similar phenomenon was also found in F2 progeny from a transgenic line which had been crossed with wild-type Arabidopsis plant, WS-O (GUS
gene inactivation frequency 9.9%). These results indicate that the foreign gene introduced into the plant was inactivated progressively in its transmission during subsequent generations and the transgenic line with multi-copies of T-DNA tended to show more increased inactivation.
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KEYWORD
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Arabidopsis thaliana, T-DNA, Agrobacterium-mediated transformation, GUS inactivation, T2, T3 generation
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